Using Genetic Means to Dissect Homologous and Heterologous Protein–Protein Interactions of PKR, the Interferon-Induced Protein Kinase

Using Genetic Means to Dissect Homologous and Heterologous Protein–Protein Interactions of PKR, the Interferon-Induced Protein Kinase

Source :

• Methods [ 1046-2023 ] ; 1998.

English descriptors

• Teeft :
  • Acad, Academic press, Agar, Agar plate, Agar plates, Amino acids, Antiproliferative response, Antiviral, Antiviral function, Appropriate restriction enzymes, Assay, Binding buffer, Binding domain, Biochem, Biol, Blue colonies, Cdna, Cdna expression library, Cdna library, Cell debris, Cell growth, Cellular, Cellular inhibitor, Cellular proteins, Cellular regulation, Chem, Clone, Clontech, Clontech laboratories, Coexpression, Cold spring harbor, Cold spring harbor laboratory press, Coli, Coli strains, Competitive inhibitor, Dimer, Dimer formation, Dimerization, Dimerization domain, Dimerization process, Dimerization properties, Direct interaction, Encoding, Eppendorf tube, Experimental systems, Falcon tube, False positives, Forms dimers, Further analysis, Fusion, Fusion protein, Fusion proteins, Gal4, Gene fusion, Genetic approaches, Genetic results, Heterotypic, Heterotypic interactions, Hf7c, High levels, Homo, Hovanessian, Important role, Indicator plates, Inhibitor, Interferon, Interferon cytokine, Internal fragments, Iptg, Katze, Kinase, Kinase activity, Lacz, Lacz expression, Lacz reporter, Lamb mutants, Library plasmids, Lysis buffer, Lysis plaques, Mathews, Meurs, Modulating kinase dimerization, Monoclonal antibody, Mrna translation, Mutant, Natl, Nonfat milk powder, Nonfunctional form, Other proteins, Overnight cultures, Overnight incubation, Pellet, Phage, Phage lytic growth, Physical association, Plaque assay, Plasmid, Plasmid selection, Positive clones, Potential mechanisms, Proc, Promoter, Protein, Protein interaction, Protein interactions, Protein kinase, Protein kinases, Pulldown assay, Repressor, Repressor activity, Repressor fusion, Repressor fusion system, Repressor system, Resistant cells, Resultant plasmids, Room temperature, Sample buffer, Second protein, Sterile toothpick, Supernatant, Transformants, Translation products, Translational control, Unpublished data, Vaccinia virus, Viral, Viral infection, Viral transcripts, Virol, Virology, Western blot analysis, White colony color, Yeast, Yeast colonies, Yeast strain, Yeast system, Yeast systems.

Abstract

• https://api.istex.fr/ark:/67375/6H6-QNF9XQ87-S/fulltext.pdf

Affiliations:

• États-Unis
• Washington (État)
• Seattle
• Université de Washington

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